Category: General
Sep 15, 2016

95% QE Back Illuminated Scientific CMOS Camera

Scitech is proud to introduce the new Prime 95B Back Illuminated Scientific CMOS (sCMOS) camera by Photometrics with 95% Quantum Efficiency (QE) making it the ideal choice for Super-Resolution Microscopy, Confocal Microscopy, Single Molecule Fluorescence and Light Sheet Microscopy

The Photometrics Prime 95B sCMOS camera is the first sCMOS camera to offer 95% Quantum Efficiency (QE) and Back Illumination (BI) in the same camera.

The Prime 95B’s sensor converts up to 95% of incident photons into a measurable signal. Unlike microlens approaches to increasing QE, which lose effectiveness as objective magnification is increased, Prime 95B’s BI sensor brings light into the pixel photodiode from behind, avoiding structures that reflect or absorb light. When combined with large 11μm pixels, the Prime 95B camera can deliver over 300% more signal than other sCMOS cameras at 100X magnification.

More importantly, the Prime 95B sCMOS camera outperforms EMCCD cameras - with no excess noise that negates the benefit of using a high QE sensor, and additional limitations from EM gain calibration, stability, expense, and sensor lifetime. With a true 16-bit dynamic range, the Prime 95B sCMOS camera easily accomplishes what EMCCD can not – it detects weak and bright signals within the same image with photon-noise limited performance.

The extreme sensitivity not only allows fainter signals to be detected, it provides the flexibility to increase frame rates, or turn down the excitation intensity to reduce cellular photo-damage. Yet Prime 95B maintains the same high frame rates, field-of-view and extremely low read noise that has made sCMOS so popular for live-cell imaging

Key features include 95% QE,16 bit dynamic range, large11μm x 11μm Pixel Area, 1.3e- Read Noise (rms), 41fps @ 16-bit / 82fps @ 12-bit, regulated air cooling to -10 deg C, single cable connection rather than a dual camera link, large field of view, C Mount interface, multiple expose out triggering and SMART streaming.

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