Active Illumination

Active Illumination (AI) describes a rapidly evolving range of optical techniques with an increasing impact on scientific enquiry and experimentation. All AI techniques target optical radiation at user-defined region(s) of the specimen and use optical imaging to observe, and often to measure, the impact of the “perturbation”.

Model Description
Mosaic Simultaneously illuminate multiple regions of interest.
MicroPoint Diffraction limited illumination from a pulsed dye-laser.
FRAPPA Unique design for galvo-scanned photobleaching and activation.



Mosaic

Simultaneous illumination of multiple regions of interest in real time and with zero delta acquisition time

Mosaic is the perfect tool for active illumination applications such as Optogenetics, optophysiology, photobleaching/activation and uncaging. This patented combination of digital mirror device used with a microscope delivers high speed targeting of complex light patterns to your sample. For optogenetics you can even combine complimentary activating and inhibitory wavelengths at the same time for tight spatial target protein control.

  • Unlimited flexibility in shape, size, complexity of illumination mask
  • Simultaneous illumination of multiple regions of interest
  • Precise illumination of areas of interest that protects the target specimen
  • Zero delta acquisition time for true digital excitation
  • Complementary illumination option enables on and off control

More information at Andor's website: click here

Download Active Illumination Catalogue (8MB)






Micropoint

Simultaneous and precise illumination and ablation

MicroPoint provides a flexible and field-proven tool for photo-stimulation. Supplied with a patented compact, pulsed nitrogen pumped tunable dye laser it is capable of ablation, bleaching and uncaging over a wavelength range of 365 to 656 nm.

  • Simultaneous laser delivery, microscope viewing and image acquisition
  • Low maintenance with fiber optic delivery that maintains alignment when system is moved
  • Quick set-up with manual beam positioning or automatic pattern generation
  • User control of ablation and illumination plane provided by z-axis telescope
  • Precise control of energy provided by motorized variable attenuator slide

More information at Andor's website: click here

Download Active Illumination Catalogue (8MB)






FRAPPA

Share all laser lines for photo stimulation and imaging

Andor’s FRAPPA uses a dual galvanometer scan head to provide a computer-steered laser beam delivery system. It can be configured in line with a CSU and/or camera and operates in two modes CSU By-Pass Mode and Frap Mode.

  • "Bypass" mode provides 1:1 relay imaging for "in-line" configuration
  • "FRAPPA" mode performs laser scanning via imaging C-port
  • Diffraction limited spot size ~0.6 μm @488 nm FWHM
  • "In-line" operation enables use of all laser lines for FRAPPA actions
  • Mode switching in < 10 ms

More information at Andor's website: click here

Download Active Illumination Catalogue (8MB)