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Calcium Imaging


Andor Technology

iXon EMCCD Camera Series

Driving the Absolute Best from EMCCD Camera Technology

With the iXon EMCCD cameras, Andor have delivered a dedicated, truly high-end, yet accessible ultrasensitive scientific camera platform, designed specifically to drive the absolute best from EMCCD technology across all critical performance specs and parameters

Andor's iXon design ensures the absolute highest sensitivity from a quantitative scientific digital camera, particularly under fast frame rate conditions. The NEW iXon Ultra innovation has raised the bar considerably, pushing frame rates to over 60% faster, the data stream transmitted through a convenient plug and play USB 2.0 interface!

Andor's proven UltraVac™ vacuum technology, carrying a 7 year warranty, is critical to ensure both deep cooling and complete protection of the sensor.

Fastest Frame Rates Available iXon Ultra delivers >60% faster frame rates than closest
EX2 Technology Extended QE response, beyond standard back-illuminated.
Market leading TE cooling to -100 °C Critical for elimination of darkcurrent detection limit.
Cropped Sensor Mode Specialized acquisition mode for continuous imaging with fastest possible temporal resolution (e.g. iXon Ultra offers 595 fps with 128 x 128 cropped region). Use with microscope accessory.
Fringe Suppression Technology Reduced etalonging in NIR.
OptAcquire™ Optimize the highly flexible iXon for different application requirements at the click of a button.
Count Convert Quantitatively capture and view data in electrons or incident photons. Applied either in real time or post-processing, Count Convert does this important conversion for you.
RealGain™ Absolute EMCCD gain selectable directly from a linear and quantitative scale.
EMCAL™ Patented user-initiated self-recalibration of EM Gain.
Superior EM Quantitative Stability and Baseline Clamp Essential for quantitative accuracy of dynamic measurements, effective even with new iXon Ultra 17MHz readout speed.
Minimal Clock-Induced Charge Unique pixel clocking parameters, yielding minimized spurious noise floor.
UltraVac™ Critical for sustained vacuum integrity and to maintain unequalled cooling and QE performance, year after year. 7 year vacuum warranty.
Spurious Noise Filter Intelligent algorithms to filter clock induced charge events from the background. Real time or post-processing.
iCam Unique innovation that empowers the iXon to operate with market-leading acquisition efficiency through 3rd party live cell microscopy software.
Enhanced Photon Counting Modes Intuitive single photon counting modes to overcome multiplicative noise. Real time or post-processing.
'2 in 1' flexibility (897 and 888 model types) EMCCD for ultra-sensitivity at speed, conventional CCD for longer acquisitions. CCD mode now with ultra-low 3 e- noise (iXon Ultra 897)
Direct Data Access (iXon Ultra) Unique Camera Link output port to facilitate direct access to raw data for on the fly processing, ideal for applications such as adaptive optics.
USB 2.0 (iXon Ultra) True universal 'plug and play' interface to PC.

Active Pixels
Pixel Size
Frame Rate
Read Noise
iXon Ultra 897
512 x 512
16 x 16
< 1
iXon3 860
128 x 128
24 x 24
< 1
iXon3 888
1024 x 1024
13 x 13
< 1
iXon3 897
512 x 512
16 x 16
< 1

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MetaFluor Fluorescence Ratio Imaging Software

MetaFluor® Fluorescence Ratio Imaging Software is the industry standard for monitoring fluorescence ratio imaging applications, bringing microscopists closer to rapid cellular responses inherent to live cells.

MetaFluor Fluorescence Ratio Imaging Software is designed for dual-wavelength intracellular ion measurements. The system provides simultaneous display of the raw data; ratio image; graphs of intensities, ratios, and ion concentrations (e.g., calcium, sodium, potassium, chloride); and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously to provide greater insight to ion exchange and intracellular function regardless of dye loading concentrations, conditions, or emission intensities.

Toolbars, menus, wizards, and dialog boxes help move you through the image processing steps quickly. Features such as multiple image windows, flexible device control, synchronization and timing, and journals allow for automated image acquisition and analysis unlike any other system.

With MetaFluor Fluorescence Software, you customize the set-up once, then let the experiment run by itself. You are able to collect a large amount of data online and process it with either MetaFluor Software or independently using an analysis-only software version.

MetaFluor Fluorescence Ratio Imaging Software features:

  • Real time processing significantly reduces random image noise by averaging up to 256 images per time point. Background subtraction is also used to improve accuracy by correcting for stray light, camera noise, and auto-fluorescence.
  • Calibrations of intracellular ion concentrations are obtained by using the various calibration options offered: the Grynkiewicz equation (Grynkiewicz et al., 1985) and titration equation for both in situ and in vitro experiments. These calibrations can then be stored for future use.
  • Region analysis can be generated automatically or manually to monitor intensity, ratio value, or ion concentration. Measurements are then made simultaneously on all the regions of interest and update continuously on a scrolling graph, allowing you to follow dynamic changes as they occur in your living samples.
  • Interactive graphs give flexibility in the presentation of your experiment's data. MetaFluor Software enables you to click on graph traces to display a readout of the time and data value for the region nearest to the click.
  • Event mark function is useful to record when drugs or solutions are added, experimental conditions changed, triggers are received or sent, or other events occurred. You have the option to associate a timer and an alarm bell to each event. Additionally, for perfused samples, ambient conditions can be logged and tracked.
  • Journals allow you to create functions to simplify system operations, automate acquisition and device control, set variables, and sequence events. The Auto-Execute Journal command allows you to automatically run journals at specific points in the acquisition cycle.

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Light Source

Polychrome V

The Polychrome V is an ultra fast switching monochromator with a fully digital high precision galvanometer driven grating. Whether you do high speed ratio imaging experiments, GFP-imaging with a variety of fluorophores to be distinguished, FRET with the faintest signals to be detected, Photometry with very high time resolution or TIRF in combination with wide−, the Polychrome V with its optimized condensers for all major microscopes is your superior companion.


  • exceptional stability of the light output
  • DSP driven control for highest precision
  • longer life expectance of bulb and light guide
  • stand alone and more compact − no external control box or power supply needed
  • more versatile − as many wavelengths as you wish
  • UV−Vis enhanced
  • easy optical coupling to standard microscopes − also fits in your existing setup
  • continuously variable bandwidth control (optional)
  • variable intensity control 0 − 100%, in steps of 10% (optional)

Key Specifications
wavelength range 320 nm to 680 nm
lamp type 150 W Xenon high stability lamp
lamp lifetime 2000 h (average)
output power > 10mW at 470 nm with new lamp
monochromator scanner mounted diffraction grating
half-power bandwidth 15 nm
λ − switch speed up to 400 nm/ms
optical fiber UV/Vis quartz/quartz fiber, length 2 m or 3 m
interface voltage (−10 V to +10 V), RS232, trigger in and out
operating voltage 110 V − 120 V, 210 V − 240 V
size (cm) 42 x 24 x 21

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