Calcium Imaging


Andor Technology

iXon EMCCD Camera Series

With the iXon EMCCD cameras, Andor have delivered a dedicated, truly high-end, yet accessible ultrasensitive scientific camera platform, designed specifically to drive the absolute best from EMCCD technology across all critical performance specs and parameters.

Model Active Pixels Cooling Pixel Size Frame Rate Read Noise
iXon Ultra 897 512 x 512 -100 °C 16 x 16 56 < 1
iXon Ultra 888 1024 x 1024 -95 °C 13 26 < 1
iXon3 860 128 x 128 -100 °C 24 x 24 513 < 1


Legacy iXon 3 models: Legacy iXon3 897 and iXon3 888 models are still available upon request, facilitating either a preference for PCI-e interface or for direct expansion/recreation of an existing set-up.

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Andor Technology
Andor Neo - SCMOS Camera

Neo and Zyla sCMOS Cameras


Scientific CMOS (sCMOS) is a breakthrough technology that offers an advanced set of performance features that render it ideal to high fidelity, quantitative scientific measurement. The multi-megapixel sensors offers a large field of view and high resolution, without compromising read noise, dynamic range or frame rate.

Find the perfect sCMOS camera for your research - Download the sCMOS Decision Chart.

Model Active Pixels Frame Rate Dark Current
Neo 5.5 sCMOS 2560 x 2160 30 0.007
Zyla 5.5 sCMOS 2560 x 2160 100 (40 USB 3.0) 0.14
Zyla 4.2 sCMOS 2048 x 2048 100 (53 USB 3.0) 0.14



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MetaFluor Fluorescence Ratio Imaging Software

MetaFluor® Fluorescence Ratio Imaging Software is the industry standard for monitoring fluorescence ratio imaging applications, bringing microscopists closer to rapid cellular responses inherent to live cells.

MetaFluor Fluorescence Ratio Imaging Software is designed for dual-wavelength intracellular ion measurements. The system provides simultaneous display of the raw data; ratio image; graphs of intensities, ratios, and ion concentrations (e.g., calcium, sodium, potassium, chloride); and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously to provide greater insight to ion exchange and intracellular function regardless of dye loading concentrations, conditions, or emission intensities.

Toolbars, menus, wizards, and dialog boxes help move you through the image processing steps quickly. Features such as multiple image windows, flexible device control, synchronization and timing, and journals allow for automated image acquisition and analysis unlike any other system.

With MetaFluor Fluorescence Software, you customize the set-up once, then let the experiment run by itself. You are able to collect a large amount of data online and process it with either MetaFluor Software or independently using an analysis-only software version.

MetaFluor Fluorescence Ratio Imaging Software features:

  • Real time processing significantly reduces random image noise by averaging up to 256 images per time point. Background subtraction is also used to improve accuracy by correcting for stray light, camera noise, and auto-fluorescence.
  • Calibrations of intracellular ion concentrations are obtained by using the various calibration options offered: the Grynkiewicz equation (Grynkiewicz et al., 1985) and titration equation for both in situ and in vitro experiments. These calibrations can then be stored for future use.
  • Region analysis can be generated automatically or manually to monitor intensity, ratio value, or ion concentration. Measurements are then made simultaneously on all the regions of interest and update continuously on a scrolling graph, allowing you to follow dynamic changes as they occur in your living samples.
  • Interactive graphs give flexibility in the presentation of your experiment's data. MetaFluor Software enables you to click on graph traces to display a readout of the time and data value for the region nearest to the click.
  • Event mark function is useful to record when drugs or solutions are added, experimental conditions changed, triggers are received or sent, or other events occurred. You have the option to associate a timer and an alarm bell to each event. Additionally, for perfused samples, ambient conditions can be logged and tracked.
  • Journals allow you to create functions to simplify system operations, automate acquisition and device control, set variables, and sequence events. The Auto-Execute Journal command allows you to automatically run journals at specific points in the acquisition cycle.

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