JPK Instruments AG
JPK Instruments AG is a world-leading manufacturer of nanoanalytic instruments - particularly atomic force microscope (AFM) systems and optical tweezers - for a broad range of applications reaching from soft matter physics to nano-optics, from surface chemistry to cell and molecular biology. From its earliest days applying atomic force microscope (AFM) technology, JPK has recognized the opportunities provided by nanotechnology for transforming life sciences and soft matter research. This focus has driven JPK’s success in uniting the worlds of nanotechnology tools and life science applications by offering cutting-edge technology and unique applications expertise.
Atomic Force Microscope
Developed on the basis of unique applications expertise , the JPK NanoWizard ® AFM family addresses researchers in both classical materials science as well as in soft matter and life sciences.
Specialized solutions like stand alone tip scanning design, stability and flexibility, best AFM performance, even in liquids, innovations like HyperDrive™ or the patented DirectOverlay™ allow researchers in almost every field precise and easy work.
NanoWizard® 4 BioScience AFM
The NanoWizard® 4 BioScience AFM is the only AFM system on the market which is designed for optimal use in liquid and comes with a vapor barrier, encapsulated piezos and a variety of dedicated liquid cells for applications ranging from single molecules to living cells. Of course, one can use the system in air or controlled environment too.
NanoWizard®4 Bioscience AFM - for applications in Soft Matter and Life Science research reaching from biophysics and cell biology to surface science and biomedicine
NanoWizard® 4 ULTRA Speed AFM
Fast-Scanning and Super-Resolution AFM on inverted microscope enabling tracking of changes in samples in real time
NanoWizard® Ultra Speed AFM
NanoWizard® 3 NanoScience AFM
The new NanoWizard® 3 NanoScience AFM is perfect for applications ranging from imaging single molecules, polymers, nanoparticles, and materials to electrical, optical, electrochemical and mechanical measurements in controlled environments.
NanoWizard®3 Nanoscience AFM
NanoWizard® 3 NanoOptics AFM
The new NanoWizard® 3 NanoOptics AFM is optimized for a broad range of applications ranging from nanoscale optical imaging by aperture and scattering-type SNOM to experiments involving interactions of light with the sample such as absorption, excitation, nonlinear effects and quenching.
- Comprehensive solutions for AFM and Raman spectroscopy, Tip-Enhanced Raman Spectroscopy (TERS), Aperture SNOM and Scattering-typeSNOM (sSNOM), Confocal microscopy, NanoManipulation in optical fields
- Compatible with most commercially available inverted research microscopes (Zeiss Axiovert and Axio Observer lines, Nikon TE and Ti lines, Olympus IX line and Leica DMI line)
- Unique integration with optical microscopy by tip and sample scanning design, DirectOverlay™ mode and smart engineering
- Seamless integration with inverted microscopes, Raman spectrometers, photon counting systems
JPK NanoWizard® AFM - tip scanner vs. sample scanner
JPK BioMAT™ workstation design animation
TAO™ - Tip Assisted Optics module for combined AFM and optical spectroscopy studies for applications in Raman/TERS or nanooptics
BioMAT™ Workstation - for opaque samples combining upright optical microscopy with AFM for surface science and life science
JPK Accessories - 16 pages full of ideas for a wider range of SPM-related applications.
NanoTracker™ & NanoWizard® - The powerful Optical Tweezers & AFM Combination
Extending the force range from nanoNewtons to femtoNewtons
The OT-AFM Combi-System pairs the exceptional surface force measurement and imaging capabilities of AFM with the ability of optical tweezers to apply and measure smallest forces in 3D.
The unique combination of 3D positioning, detection, and manipulation provided by OT and the high-resolution imaging and surface property characterization of AFM opens up a whole new spectrum of applications.
NanoWizard® and NanoTracker™ team up – the perfect toolbox for imaging and force applications
The combined setup fulfills the highest demands on mechanical stability, flexibility, and modularity. A specially designed OT-AFM ConnectorStage™ is the key to combining any AFM of the NanoWizard® or CellHesion® family with the NanoTracker™ optical tweezers on a research-grade inverted optical microscope.
- Imaging, positioning, and manipulation experiments from single molecules to living cells
- Measure forces in 2D and 3D, from 500fN to 10nN, on the same sample
- Fully flexible and modular design, with the widest range of modes & accessories
- Comprehensive integration with optical microscopy techniques such as TIRF or confocal
- All NanoTracker™, NanoWizard® /CellHesion® 200 systems can be combined to become a complete OT-AFM-platform
- The ConnectorStage™ combines the AFM with the tweezers hardware on all major inverted optical microscopes from Zeiss, Nikon, Olympus, and Leica
Automated Atomic Force Spectroscopy
Force spectroscopy is a single molecule technique that allows the real-time study of molecular interactions on the nanoscale. Originating from the broad field of Atomic Force Microscopy, force spectroscopy provides the necessary sensitivity to characterize biomolecular interactions such as the unfolding forces of single proteins or forces of a single chemical bond.
For the very first time, the automation of force spectroscopy makes it fast enough to deliver high quality data in short time-frames.
JPK provides the following solution:
ForceRobot® 300 - with optional temperature control and fluidics system
Optical Tweezers / 3D Particle tracking and trapping
Optical tweezers enable trapping and manipulation of nanoparticles, microparticles, and biological species in fluid media. Now, JPK's unique Nanotracker™ system extends this technology to enable measurement of interaction forces with sub picoNewton sensitivity. In addition, particles are simultaneously tracked in 3-D to quantify dynamics, viscosity, diffusion and host of other processes.
For the first time, dual beam force-sensing optical tweezers seamlessly integrate on inverted optical microscopes combining advanced optical and confocal techniques including single molecule fluorescence in a small footprint, easy to use system.
Our unique tweezers technology (also known as a Photonic Force Microscope) enables quantification of molecular, cellular and micro-rheological processes. Applications include molecular motor mechanics, binding/elasticity of DNA and proteins, cell membrane dynamics and particle uptake. JPK's Nanotracker™ is set to revolutionize research in biophysics, biochemistry, drug discovery, toxicology and many other fields.
NanoTracker JPK Multiplexing
NanoTracker fluorescent DNA Manipulation
NanoTracker™ - unique force-sensing optical tweezers and particle tracking system
Cellular Adhesion & Cytomechanics
Our CellHesion ® products quantify single cell-cell and cell-surface interactions under physiological conditions. This ground-breaking technique, known as single cell force spectroscopy (SCFS), measures the interaction forces between a living cell bound to a cantilever and a target cell, functionalised substrate or biomaterial. In parallel, cytomechanical characteristics including stiffness and elasticity can be determined.
Uniquely, specific and non-specific adhesion events are differentiated. The roles of cell adhesion molecules, extracellular matrix proteins, antibodies and drugs can be quantified to characterize processes from single integrin binding through to membrane tether extraction. Our instruments deliver new insights in developmental biology, cancer biology, immunology and biocompatibility.
We provide both a stand-alone platform to add to your inverted optical or confocal microscope and an accessory module for our powerful NanoWizard ® Atomic Force Microscope (AFM). Easy to use acquisition software combined with automated batch analysis of results accelerates research and reduces your time to publication.
CellHesion® 200 - a dedicated platform for cell adhesion and cytomechanics studies
CellHesion® Module - accessory for the NanoWizard® AFM combines cell adhesion and mechanics with AFM imaging techniques
The result of a single measurement cycle is a force vs. distance curve, which allows to determine single molecule events, the "work of removal" W, tether formation, the maximum adhesion force and viscoelastic parameters.
Peer Reviewed papers:
Natural Bactericidal Surfaces: Mechanical Rupture of Pseudomonas aeruginosa Cells by Cicada Wings
Elena P. Ivanova , * Jafar Hasan , Hayden K. Webb , Vi Khanh Truong ,
Gregory S. Watson , Jolanta A. Watson , Vladimir A. Baulin , Sergey Pogodin ,
James Y. Wang , Mark J. Tobin , Christian Löbbe , and Russell J. Crawford
Natural superhydrophobic surfaces are often thought to have antibiofouling potential due to their self-cleaning properties. However, when incubated on cicada wings, Pseudomonas aeruginosa cells are not repelled; instead they are penetrated by the nanopillar arrays present on the wing surface, resulting in bacterial cell death. Cicada wings are effective antibacterial, as opposed to antibiofouling, surfaces.
Assembly and Degradation of Low-Fouling Click-Functionalized Poly(ethylene glycol)-Based Multilayer Films and Capsules
Melissa K. M. Leung , Georgina K. Such , Angus P. R. Johnston , Dhee P. Biswas , Zhiyuan Zhu , Yan Yan , Jean-François Lutz , and Frank Caruso
Small 7 (2011)1075-85
Nano-/micrometer-scaled films and capsules made of low-fouling materials such as poly(ethylene glycol) (PEG) are of interest for drug delivery and tissue engineering applications. Herein, the assembly and degradation of low-fouling, alkynefunctionalized PEG (PEG Alk ) multilayer films and capsules, which are prepared by combining layer-by-layer (LbL) assembly and click chemistry, are reported. A nonlinear, temperature-responsive PEG Alk is synthesized, and is then used to form hydrogen-bonded multilayers with poly(methacrylic acid) (PMA) at pH 5. The thermoresponsive behavior of PEG Alk is exploited to tailor film buildup by adjusting the assembly conditions. Using alkyne–azide click chemistry, PEG Alk /PMA multilayers are crosslinked with a bisazide linker that contains a disulfide bond, rendering these films and capsules redox-responsive. At pH 7, by disrupting the hydrogen bonding between the polymers, PEG Alk LbL films and PEG Alk -based capsules are obtained. These films exhibit specific deconstruction properties under simulated intracellular reducing conditions, but remain stable at physiological pH, suggesting potential applications in controlled drug release. The low-fouling properties of the PEG films are confirmed by incubation with human serum and a blood clot. Additionally, these capsules showed negligible toxicity to human cells.
Reversible Shape Memory of Nanoscale Deformations in Inherently Conducting Polymers without Reprogramming
Michael J. Higgins, Willo Grosse, Klaudia Wagner, Paul J. Molino, and Gordon G. Wallace
J. Phys. Chem. B 115 (2011) 3371–3378
By using inherently conducting polymers, we introduce new shape memory functionality for stimuli-responsive polymers. The shape memory process is unique in that it utilizes electrochemical control of the polymer redox state to conceal, and temporarily store, preformed nanoscale surface patterns, which can later be recalled. Unlike classical thermoset and thermoplastic shape memory polymers, the electrochemical control does not completely perturb the low entropy state of the deformed polymer chains, thus enabling the concept of reversible transition between the permanent and temporary shapes. This is demonstrated using electrochemical-atomic force microscopy/quartz crystal microbalance to characterize the modulation of nanoscale deformations in electroactive polybithiophene films. Experimental results reveal that cation/solvent exchange with the electrolyte and its effect on reconfiguration of the film structure is the mechanism behind the process. In addition
to incorporating conductive properties into shape-memory polymers, the ability to reversibly modulate surface nanopatterns in a liquid environment is also of significant interest in tribology and biointerface applications.
Conducting polymers with immobilised fibrillar collagen for enhanced neural interfacing
Xiao Liu, Zhilian Yue, Michael J. Higgins, Gordon G. Wallace
Biomaterials (2011), doi:10.1016/j.biomaterials.2011.06.047
Conducting polymers with pendant functionality are advantageous in various bionic and organic bioelectronic applications, as they allow facile incorporation of bio-regulative cues to provide bio-mimicry and conductive environments for cell growth, differentiation and function. In this work, polypyrrole substrates doped with chondroitin sulfate (CS), an extracellular matrix molecule bearing carboxylic acid moieties, were electrochemically synthesized and conjugated with type I collagen. During the coupling process, the conjugated collagen formed a 3-dimensional fibrillar matrix in situ at the conducting polymer interface, as evidenced by atomic force microscopy (AFM) and fluorescence microscopy under aqueous physiological conditions. Cyclic voltammetry (CV) and impedance measurement confirmed no significant reduction in the electroactivity of the fibrillar collagen-modified conducting polymer substrates. Rat pheochromocytoma (nerve) cells showed increased differentiation and neurite outgrowth on the fibrillar collagen, which was further enhanced through electrical stimulation of the underlying conducting polymer substrate. Our study demonstrates that the direct coupling of ECM components such as collagen, followed by their further self-assembly into 3-dimensional matrices, has the potential to improve the neural-electrode interface of implant electrodes by encouraging nerve cell attachment and differentiation.
Skeletal muscle cell proliferatinio and differentiation on polypyrrole substrates doped with extracellular matrix components
Kerry J. Gilmore, Magdalena Kita, Yao Han, Amy Gelmi, Michael J. Higgins, Simon E. Moulton, Graeme M. Clark, Robert Kapsa and Gordon G. Wallace
Biomaterials 30 (2009) 5292-5304
Conducting polymers have been developed as substrates for in vitro studies with a range of cell types including electrically-excitable cells such as nerve and smooth muscle. The goal of this study was to optimise and characterise a range of polypyrrole materials to act as substrates for electrical stimulation of differentiating skeletal myoblasts. Although all of the polymer materials provided suitable substrates for myoblast adhesion and proliferation, significant differences became apparent under the low-serum conditions used for differentiation of primary myoblasts. The significance of the work lies in the design and control of polymer materials to facilitate different stages of skeletal muscle cell proliferation and/or differentiation, opening up opportunities for engineering of this tissue. This paper therefore constitutes not just a biocompatibility assessment but a comprehensive study of how synthesis conditions affect the final outcome in terms of cell response.
Combined AFM-Confocal Microscopy of Oil Droplets: Absolute Separations and Forces in Nanofilms
Rico F. Tabor, Hannah Lockie, Douglas Mair, Rogerio Manica, Derek Y. C. Chan, Franz Grieser, and Raymond R. Dagastine
J. Phys. Chem. Lett. 2 (2011) 961-965
Quantitative interpretation of the dynamic forces between micrometer-sized deformable droplets and bubbles has previously been limited by the lack of an independent measurement of their absolute separation. Here, we use in situ confocal fluorescence microscopy to directly image the position and separation of oil droplets in an atomic force microscopy experiment. Comparison with predicted force vs. separation behavior to describe the interplay of force and deformation showed excellent agreement with continuum hydrodynamic lubrication theory in aqueous films less than 30 nm thick. The combination of force measurement and 3D visualization of geometric separation and surface deformation is applicable to interactions between other deformable bodies.
The effect of unlocking RGD-motifs in collagen I on pre-osteoblast adhesion and differentiation
Anna V. Taubenberger, Maria A. Woodruff, Huifen Bai, Daniel J. Muller, Dietmar W. Hutmacher
Biomaterials 31 (2010) 2878-2835
Denaturation of extracellular matrix proteins exposes cryptic binding sites. It is hypothesized that
binding of cell adhesion receptors to these cryptic binding sites regulates cellular behaviour during tissue
repair and regeneration. To test this hypothesis, we quantify the adhesion of pre-osteoblastic cells to
native (Col) and partially-denatured (pdCol) collagen I using single-cell force spectroscopy. During early
stages of cell attachment (<=180 s) pre-osteoblasts (MC3T3-E1) adhered significantly stronger to pdCol
compared to Col. RGD (Arg-Gly-Asp)-containing peptides suppressed this elevated cell adhesion. We
show that the RGD-binding alpah5beta1- and alpha(v)-integrins mediated pre-osteoblast adhesion to pdCol, but not to Col. On pdCol pre-osteoblasts had a higher focal adhesion kinase tyrosine-phosphorylation level that
correlated with enhanced spreading and motility. Moreover, pre-osteoblasts cultured on pdCol showed
a pronounced matrix mineralization activity. Our data suggest that partially-denatured collagen exposes
RGD-motifs that trigger binding of a5b1- and av-integrins. These integrins initiate cellular processes that
stimulate osteoblast adhesion, spreading, motility and differentiation. Taken together, these quantitative
insights reveal an approach for the development of alternative collagen I- based surfaces for tissue
Single-molecule biophysics: at the interface of biology, physics and chemistry
Ashok A. Deniz, Samrat Mukhopadhyay and Edward A. Lemke
J. R. Soc. Interface (2008) 5, 15–45
Single-molecule methods have matured into powerful and popular tools to probe the complex
behaviour of biological molecules, due to their unique abilities to probe molecular structure,
dynamics and function, unhindered by the averaging inherent in ensemble experiments. This
review presents an overview of the burgeoning field of single-molecule biophysics, discussing
key highlights and selected examples from its genesis to our projections for its future.
Following brief introductions to a few popular single-molecule fluorescence and manipulation
methods, we discuss novel insights gained from single-molecule studies in key biological areas
ranging from biological folding to experiments performed in vivo.
Keywords: single-molecule fluorescence; force; FRET; tracking; AFM; optical tweezers