MetaMorph Microscopy Automation & Image Analysis Software
MetaMorph® Microscopy Automation & Image Analysis Software is the industry standard for automated microscope acquisition, device control, and image analysis, bringing microscopists greater understanding of cell morphology, function, and behavior for over 25 years.
Device Control: MetaMorph Software is the ideal "glue" for easily integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation. Each custom system can be uniquely suited for virtually any experimental paradigm. System integration is made even easier by supporting all major brands of automated microscopes and scientific CCD cameras. This means MetaMorph Software can be used as a common software platform within a core facility or laboratory minimizing maintenance, training, and support of customers and end-users.
Analysis: Acquiring the image is only the first step. MetaMorph Software provides all the tools needed to perform meaningful analysis of acquired images. Common tools for thresholding, filtering, and feature extraction are included as one would expect. However, MetaMorph Software provides other more sophisticated tools such as the integrated morphometry analysis (IMA) module. IMA allows objects to be measured and separated into user-definable classes, based on any combination of measured morphometric parameters, such as shape, size, or optical density. A point-and-click interface, linking images and spreadsheet results, makes it easy to choose representative objects for classification and measurement.
MetaMorph Software also offers many user-friendly application modules for biology-specific analysis such as cell signaling, cell counting, and protein expression. Each application module features a dedicated dialog box with intuitive setting selections, improved segmentation through adaptation to local content, as well as both field and cell-by-cell data logging. After analysis is complete, users can interactively view tabular and image results side by side for their cellular imaging application.[link to application module product description page]
Automation: Often the hardest task with any imaging system is automating complex, multi-parametric experimental protocols. MetaMorph Software handles this elegantly with its multi-dimensional aquisition (MDA) module. MDA guides the user through the setup of each acquisition dimension (time, z, fluorescent channels, stage position). Once a particular acquisition is completed through the MDA interface, all settings can be stored and recalled for later use making it easy to perform repeated operations.
For those who want to further automate acquisition, processing, and analysis routines, MetaMorph Software features sophisticated and powerful macros called journals. Journals record and save a series of tasks without the need for programming. A graphical editor allows for easy creation and editing of journals using a drag-and-drop interface.
MetaMorph Software is a modular platform that allows you to select only the components you need. Modules are available for:
- Camera control and acquisition
- Microscope automation and control
- Sample positioning and specimen illumination
- Application modules to make complex, repetitive tasks easier to perform
MetaMorph Software Application Modules
Combine ease-of-use & flexibility to accommodate automated image analysis for hundreds of applications in both research and high throughput environments.
A modular toolbox for image analysis, the suite of MetaMorph® Software Application Modules enable both novice and expert users to set up hundreds of assays in minutes without compromising the mature image processing algorithms required to address complex applications. The Application Modules provide an interactive environment to determine features of interest based on size, intensities, and distances. Some modules handle very specific and challenging assays (e.g., neurite outgrowth) while others (e.g., multi-wavelength cell scoring) can be configured to quantify dozen of different cellular events. To further tailor analysis to your research-oriented goals, journals can be amended to Application Modules, allowing to customize any analysis.
- 12 Application Modules allow MetaMorph Software users to automate common analysis routines and accommodate 100's of applications (see the applications tab).
- Interactive Environment guides users through the determination of features of interest.
- Adaptive Background Correction mitigates poor sample quality while improving image segmentation.
- Field-by-Field Data, Cell-by-Cell Data, and Segmentation Overlays are made available to ensure in-depth downstream analysis such as gating cell populations or reviewing accuracy of segmentation.
- Customization of Application Modules for research and assay development can easily be achieved by amending journals to Application Modules (Note: the resulting analysis will not be compatible with analysis with the MetaXpress PowerCore Software).