Optical Activation, Stimulation and Imaging
Mightex Systems, with operations based in Toronto, Canada, and in the Silicon Valley, California, is a leading supplier of photonic products and services for a broad spectrum of applications including industrial imaging, bio-photonic solutions, LED illumination, machine vision, life & health sciences, and industrial test & measurement etc.
OASIS In Vivo Platform - Modular Macroscope and Microscope
Optical Activation, Stimulation & Imaging System
- Translates and Rotates for Live Animal Research
- Simultaneously Image & Deliver Wide-field and/or Pattern Illumination
- Macro or Microscopic Optical Assembly
- Modular Platform Tailors to any Experimental Application
- Excellent System for:
- In Vivo Optogenetics
Mightex's Optical Activation, Stimulation and Imaging System (OASIS) is designed specifically to meet the growing demand to conduct experiments on live animals, when system flexibility is a primary consideration and a large Field of View (FoV) is usually required. In Optogenetics, for example, in vivo patterned illumination on rodents, monkeys and other animals is the next step towards the ultimate goal of being able to record and control the activity of each neuron in the brain of behaving animals at a single cell and ms resolution. To achieve patterned illumination for in vivo applications, the OASIS features Mightex’s market-leading Polygon400 patterned Illuminator to deliver light to a specified area of the specimen while leaving neighboring areas untouched.
Whereas traditional microscopes are a fixed system with limited flexibility, OASIS is designed to provide excellent reconfigurability/flexibility and can be configured to work around a fixed specimen such as a sedated live animal. A heavy-duty precision XYZ stage is mounted on the support structure and the OASIS optical assembly is in turn mounted on the XYZ stage. The XYZ stage controls the lateral motion as well as focus of the system. A pan/tilt mechanism add-on is also available to orient the OASIS relative to the live animal. Motorized versions of both the XYZ stage and tilt mechanism are available.
OASIS Implant - Deep-Brain Imaging & Stimulation System for Freely Behaving Animals
- In vivo all-optical recording and manipulatiion of neural circuit activity;
- Sub-cellular resolution, high-quality imaging for quantitative analysis;
- Multi-wavelength illumination;
- Patterned stimulation with sub-cellular targeting capability;
- Super-light head mounted fixture, 0.3g;
- Modular system design, reconfigurable for different applications.
Mightex's OASIS Implant is a ground-breaking microendoscopic system that is capable of doing deep-brain simultaneous all-optical recording and manipulation of neural circuit activity with cellular resolution, on freely behaving animals. Compatible with high-sensitivity research-grade cameras, OASIS Implant enables researchers to acquire far superior quality deep-brain images & videos, compared to the relatively low-quality head-mounted cameras that have recently been introduced into the market. In addition, with multiple illumination ports, OASIS Implant is equipped with both wide-field and patterned illumination capabilities (when combined with Mightex's market-leading Polygon400) and, moreover, it is also capable of doing multi-wavelength imaging/stimulation which cannot be done with the existing head-mounted cameras. More importantly, since both the deep-brain imaging and the deep-brain stimulation are delivered through an Imaging Fiber bundle, the overall weight of the head-mounted fixture is as little as 0.3g (compared to approximately 2g with a head-mounted camera), making OASIS Implant an ideal research tool for in vivo live animal experiments.
In addition to many other applications, OASIS Implant enables bioscientists to do the following:
- Deep brain calcium imaging in freely behaving animals;
- Deep brain patterned optogenetic stimulation with single-cell or sub-cellular resolution;
- Simultaneous deep brain stimulation and imaging.
Patterned Illumination Systems
Many applications (such as optogenetics, photoactivation, and photostimulation etc) call for precise spatial, temporal and/or spectral control of light. For instance, optogenetics is a technology that allows one to investigate behaviours of neurons and their circuits, by precisely delivering light - of different colors and with high spatial and temporal resolutions - to stimulate/silence selected neurons. In addition, there may also be a need to control neural activity independently and/or simultaneously at different locations. Mightex’s Polygon400 Patterned Illuminators are specifically designed for such applications.
Optogenetics & Electrophysiology
Optogenetics is a technology that allows targeted, fast control of precisely defined events in biological systems, by delivering optical control at the speed (millisecond scale) and with the precision (cell type–specific) required for biological processing.
As far as optogenetics applications are concerned, LEDs are able to simultaneously meet both the speed and the spatial precision requirements mentioned above. In addition, compared to lasers, LEDs excel in almost every respect: they are cheaper, smaller, more reliable, and easier to control.
Mightex has developed a range of Light Delivery Systems for optogenetics
BioLED Sources & Controllers
Mightex BLS-series BioLED light delivery systems are modularized fully-customizable turn-key solutions for optogenetics, fluorescence excitation, and other bioscience applications. Precisely-timed and high-intensity light pulses are required in optogenetics experiments to activate channelrhodopsins (ChR2, ChR1 etc.) and halorhodopsins (NpHR) in order to excite and inhibit neurons. To meet these requirements, Mightex has developed a proprietary “IntelliPulsing” technology to allow BLS-series sources to output significantly higher power in pulse mode than what the LEDs are rated for in CW mode.