FEMTO3D ATLAS – All-in-one 3D Microscope for 2-Photon Microscopy
Scitech is proud to announce they are now the official distributors in Australia and New Zealand for Femtonics Ltd. – specialist manufacturers of two-photon laser scanners.
The Innovation Award winner FEMTO3D Atlas combines high-tech science and engineering in 3D measurements. It implements and goes beyond the traditional galvo and resonant scanner-based imaging functions and combines them with a unique fast 3D imaging feature, providing an all-in-one solution in two-photon microscopy. The FEMTO3D Atlas enables its users to scan cells and neuronal, dendritic, or other biological processes in 3D, up to a million times faster than classical scanning methods, at a preserved two-photon resolution – making it the perfect instrument for Network and Dendritic Imaging, Behavioural Studies and Optogenetics.
Neural networks consist of thousands of neurons dispersed in 3D space, often across many cortical layers of the brain tissue. 3D random-access scanning performed by AO (acousto-optic) technology combined with two-photon microscopy is the best choice to reveal the dynamics of the neural networks while processing information. It enables recording of neuronal activity from cell populations consisting of up to thousands of cells in vivo. The 3D anti-motion technology combined with new scanning methods united in FEMTO3D Atlas provides a flexible solution to gather motion-corrected data in large volumes at high framerates during behaviour.
Available both as a standalone microscope or as a 3D imaging extension for various types of existing systems. Other features include:
- Simultaneous read out of neural activity on both the somatic and dendritic scales
- Increased measurement speed and signal-to-noise ratio by several orders of magnitude
- 3D Network imaging of over 2000 soma distributed in 3D
- Imaging of dendritic branches through layers
- Simultaneous imaging of over thousands of spines
- Unique, flexible scanning methods supporting in vivo and in vitro measurements
- In vivo real-time 3D motion correction
- High-speed 3D random-access ROI scanning: scanning speed up to 100 kHz to any points in 3D
- High-speed raster scanning: 40 fps at 510×510, and even 3000 fps at reduced FOV
- Improved excitation efficiency for large 3D scanning volume with GECIs
- Automatic wavelength tuning between 750 – 1050 nm
- Functional imaging correlated with electrophysiological data
- Calcium and voltage imaging during behavior
- 3D random-access photo stimulation for optogenetics